I am a Project Manager working in Cell and Gene therapy space. In my current job, I coordinate activities in Research and Development, where I plan, develop and manage Budget, resource and timelines for pipeline development by taking input from a team of scientists. I also have experience in planning, execution and management of manufacturing projects for clients in a GMP Cell and Gene therapy facility where I coordinated activities across various functions (MFG, QC, QA, SCM) and ensured execution of projects on time in full. Prior to this I have extensive research experience in Molecular biology, Biochemistry and Molecular epidemiology. Looking for opportunities where I can apply my technical expertise and project management skills to achieve my prospective employer’s goals.
Himani Kukreti, PhD
Project Manager
Research & Development,
Nuevocor Pte Ltd
Phone:
+65 91003881
Email:
EXPERIENCE
May 2017-Nov 2017
Post Doctoral Research Fellow
Department of Dentistry,
University of Toronto, Canada
Project: ‘Investigation of adherence of stem cells on endotoxin contaminated dentin’ - Funded by American Association of Endodontists Foundation, USA
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Contributed to a team of endodontists studying adherence of stem cells on endotoxin contaminated dentin and analysing efficacy of Dexamethasone releasing nanoparticles to rescue the effect
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Found that endotoxin inhibits adherence of viable stem cells on dentin and can be overcome by conditioning dentin with Dexamethasone releasing nanoparticles
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Provided substantial evidence on efficacy of these nanoparticles and their potential use in regenerative endodontic procedures
April 2014-April 2015
Post Doctoral Research Fellow
School of Biological Sciences,
Nanyang Technological University, Singapore
Project: ‘Identification of circulating microRNA in sarcopenia’ - Funded by National Research Foundation, Singapore.
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Contributed to a team of clinicians and scientists studying age related skeletal muscle loss
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Personally selected test and control human serum samples and completed molecular analysis to correlate circulating microRNAs with age related muscle loss.
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Identified four circulating microRNAs having potential to be used as diagnostic biomarkers for sarcopenia
Nov 2009- April 2014
Post Doctoral Research Fellow
Department of Biochemistry,
National University of Singapore, Singapore
Project: ‘Investigation of epigenetic mechanisms in Dexamethasone induced skeletal muscle cachexia’ - Funded by Biomedical Research Council, Singapore
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Explored molecular mechanism of skeletal muscle atrophy caused by anti-inflammatory and immunosupressive drug - Dexamethasone
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Deciphered a microRNA-1 mediated mechanism causing muscle wasting by targeting Heat Shock Protein 70 which eventually leads to activation of ubiqutin protein ligases- MURF and Atrogin-1, responsible for degradation of myosin heavy chain
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Published the study in Journal of Biological Chemistry
May 2005- September 2010
Doctoral Fellow
Department of Biochemistry & Biotechnology,
National Center for Disease Control, India
Project: ‘Molecular epidemiology of outbreak associated strains of Dengue Viruses’ - Funded by Council of Scientific & Industrial Research, India
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Designed study, collected and analysed human blood samples, did virus isolation and molecular genotyping of isolated strains of Dengue Viruses
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Identified DEN-3 as dominant genotype responsible for 2006 Dengue outbreak in Delhi.
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Identified co-circulating DEN-1 to be next potential outbreak causing Dengue Virus
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Published results from the study as 3 first author international publications
August 2023-Present
Project Manager, R&D
Research and Development
Nuevocor Pte Ltd, Singapore
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Manage Projects running under Discovery
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Manage contract research work by liaising with vendors
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Proactive risks and issues management
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Discovery timeline, resource and data management
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Budget planning for projects
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Coordinate regular project meetings
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Agenda development and sharing minutes
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Tracking Key project deliverables
June 2019-July 2022
Program Manager, Cell and Gene therapy
Cell and Gene Therapy
Lonza Bioscience Pte Ltd, Singapore
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Oversee smooth execution of Customer projects
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Run Customer and internal project meetings
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Pre-campaign readiness and Post-campaign reviews
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Proactive risks and issues management
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Act as interface between customer, operations and commercial teams
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forecasting revenues
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Collaborate with all functions- SCM, QA, QC, MSAT
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Report progress of project in customer meetings
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Good Manufacturing Practices
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Good Documentation Practices
May 2017-Nov 2017
Post Doctoral Research Fellow
Department of Dentistry,
University of Toronto, Canada
Project: ‘Investigation of adherence of stem cells on endotoxin contaminated dentin’ - Funded by American Association of Endodontists Foundation, USA
-
Contributed to a team of endodontists studying adherence of stem cells on endotoxin contaminated dentin and analysing efficacy of Dexamethasone releasing nanoparticles to rescue the effect
-
Found that endotoxin inhibits adherence of viable stem cells on dentin and can be overcome by conditioning dentin with Dexamethasone releasing nanoparticles
-
Provided substantial evidence on efficacy of these nanoparticles and their potential use in regenerative endodontic procedures
April 2014-April 2015
Post Doctoral Research Fellow
School of Biological Sciences,
Nanyang Technological University, Singapore
Project: ‘Identification of circulating microRNA in sarcopenia’ - Funded by National Research Foundation, Singapore.
-
Contributed to a team of clinicians and scientists studying age related skeletal muscle loss
-
Personally selected test and control human serum samples and completed molecular analysis to correlate circulating microRNAs with age related muscle loss.
-
Identified four circulating microRNAs having potential to be used as diagnostic biomarkers for sarcopenia
Nov 2009- April 2014
Post Doctoral Research Fellow
Department of Biochemistry,
National University of Singapore, Singapore
Project: ‘Investigation of epigenetic mechanisms in Dexamethasone induced skeletal muscle cachexia’ - Funded by Biomedical Research Council, Singapore
-
Explored molecular mechanism of skeletal muscle atrophy caused by anti-inflammatory and immunosupressive drug - Dexamethasone
-
Deciphered a microRNA-1 mediated mechanism causing muscle wasting by targeting Heat Shock Protein 70 which eventually leads to activation of ubiqutin protein ligases- MURF and Atrogin-1, responsible for degradation of myosin heavy chain
-
Published the study in Journal of Biological Chemistry
May 2005- September 2010
Doctoral Fellow
Department of Biochemistry & Biotechnology,
National Center for Disease Control, India
Project: ‘Molecular epidemiology of outbreak associated strains of Dengue Viruses’ - Funded by Council of Scientific & Industrial Research, India
-
Designed study, collected and analysed human blood samples, did virus isolation and molecular genotyping of isolated strains of Dengue Viruses
-
Identified DEN-3 as dominant genotype responsible for 2006 Dengue outbreak in Delhi.
-
Identified co-circulating DEN-1 to be next potential outbreak causing Dengue Virus
-
Published results from the study as 3 first author international publications
EDUCATION
2023
Project Management Professional
Project Management Institute
2005-2010
PhD (Microbiology)
Guru Gobind Singh Indraprastha Universtiy, Delhi, India
2002-2004
Master's Degree (Biotechnology)
HNB Garhwal Univeristy, India
1999-2002
Bachelor's degree (Biology)
Delhi University, Delhi, India
SKILLS
Cell and Gene Therapy
Biopharmaceutical
Manufacturing
Good Manufacturing Practices
Good Documentation Practices
Stem Cells
AWARDS & PRESENTATIONS
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Won Travel award to present my work on,‘Molecular sub typing of dengue viruses that dominated northern India causing major outbreak during post-monsoon months of 2006.’ at the Pan-American Clinical Virology Symposium 2007 held at Florida, USA.
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Won second prize for poster presentation, ‘Tracing microevolution of DENV-3 strains circulating in North India during 2003-2006 using bioinformatics.’ at the International Workshop on “Molecular Epidemiology and Immunology of Malaria and other Vector Borne Diseases” 2007 held at Jabalpur, India.
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‘Activin A-induces atrophy in C2C12 myotubes by inhibiting mTOR mediated protein synthesis.’ Poster presentation at EMBO workshop on Molecular mechanisms of muscle growth and wasting in health and disease 2013 held at Ascona, Switzerland.
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‘Regulation of myomiR-1 in skeletal muscle wasting.’ Poster presentation at ‘Molecular mechanisms of muscle growth and wasting in health and disease’ 2011 held at Ascona, Switzerland.
PUBLICATIONS
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Kukreti H, K Amuthavalli. 2020. MicroRNA‐34a causes ceramide accumulation and effects insulin signaling pathway by targeting ceramide kinase (CERK) in aging skeletal muscle.Journal of Cellular Biochemistry. https://doi.org/10.1002/jcb.29312
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Kukreti H, F.‐C. Li, K. Singh, R. Sodhi, A. Kishen. 2020. Efficacy of bioactive nanoparticles on tissue‐endotoxin induced suppression of stem cell viability, migration and differentiation. International Endodontic Journal. https://doi.org/10.1111/iej.13283
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Ge X, Sathiakumar D, Lua BJ, Kukreti H, Lee M, McFarlane C. 2016. Myostatin signals through miR-34a to regulate Fndc5 expression and browning of white adipocytes. International Journal of Obesity (London ) Jan;41(1):137-148
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Sharma M, , Kukreti H, Bonala S, Srinivasan S, McFarlane C. 2015. Myostatin: expanding horizons. International Union of Biochemistry and Molecular Biology Aug;67(8):589-600. Review.
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Sharma M, Juvvuna PK, Kukreti H, McFarlane C. 2014. Mega roles of microRNAs in regulation of skeletal muscle health and disease. Frontiers in Physiology Jun 26;5:239. Review.
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Kukreti H, Amuthavalli K, Harikumar A, Sathiyamoorthy S, Feng PZ, Anantharaj R, Tan SLK, Lokireddy S, Bonala S, Sriram S, McFarlane C, Kambadur R, Sharma M. 2013. Muscle-specific microRNA1 (miR1) targets heat shock protein 70 (HSP70) during Dexamethasone mediated atrophy. Journal of Biological Chemistry 288(9):6663–78.
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Kukreti H, Mittal V, Chaudhary A, Rautela RS, Kumar M, Chauhan S, Bhat S, Chhabra M, Bhattacharya D, Pasha ST, Seth DK, Gomber S, Lal S, Rai A. 2010. Continued persistence of a single genotype of dengue virus type-3 (DENV-3) in Delhi, India ever since its re-emergence during the current decade. Journal of Micorbiology, Immunology and Infection 43(1):53-61.
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Kukreti H, Dash PK, Parida M, Chaudhary A, Saxena P, Rautela RS, Mittal V, Chhabra M, Bhattacharya D, Lal S, Rao PVL, Rai A. 2009. Phylogenetic studies reveal existence of multiple lineages of a single genotype of DENV-1 (genotype III) in India during 1956-2007. Virology journal 6(1): 1.
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Kukreti H, Chaudhary A, Rautela R S, Anand R, Mittal V, Chhabra M, Bhattacharya D, Lal S, Rai A. 2008. Emergence of an independent lineage of dengue virus type 1 (DENV-1) and its co-circulation with pre-dominant DENV-3 during 2006 dengue fever outbreak in Delhi. International Journal of Infectious Diseases 12(5):542-549.
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Chaudhary A, Kukreti H, Pasha S T, Gupta S, Kumari M, Khare S, Lal S, Rai A. 2008. Impact of HIV on genomic variability in the 5'UTR of HCV in Indian patients with HCV/HIV co-infection. Intervirology 51(4):224-229.
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Naqvi RA, Hussain A. Raish M, Kukreti H, Chauhan M, Basir SF, Deo SVS, Shukla NK, Pasha ST, Husain SA. 2008. Methylation fingerprint analysis of MMR genes (hmlh1 and hmsh2) in Indian breast cancer patients: Potential markers to identify locally advanced breast cancer. Human pathology 39(5):633-794.
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Naqvi RA, Hussain A. Raish M, Kukreti H, Noor A, Ahamd S, Deo SVS, Shukla NK, Pasha ST, Husain SA. 2008. Pinpointing of methylation fingerprints of an imperative gene pair (FHIT and p16 genes) in Indian female breast cancer patients: A study towards better diagnosis of early and locally advanced stages of breast cancer. DNA and Cell Biology 27(9):463-465.
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Kukreti H & Singh J. 2005. Induction of heat shock proteins in groundnut (Arachis hypogaea) seedlings. Advances in Plant Sciences 18(2):493-496.
